Mechanism of nuclear DNA replication in radicles of germinating cotton
- 1 September 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (9) , 3220-3223
- https://doi.org/10.1073/pnas.73.9.3220
Abstract
The mechanism of nuclear DNA replication in radicles of germinating cotton (Gossypium barbadense) was investigated. Pulse-labeling with [3H]thymidine indicates that replication intermediates are of small MW (4-10 S) and behave as if single-stranded. Prolonged labeling indicates that intermediates are of discrete size, suggesting a mechanism of discontinuous replication. EM of nuclear DNA demonstrates a complex architecture which may include eye forms and forks similar to those reported in prokaryotes and animal systems. The possible universality of DNA replication mechanisms is discussed.Keywords
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