Liquid Chromatographic Separation with Electrochemical Detection of the Phenylthiohydantoin and Methylthiohydantoin Derivatives of Amino Acids

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Abstract
Phenylthiohydantoin (PTH) and methylthiohydantoin (MTH) derivatives of the common amino acids were examined for electroactivity on platinum and gold electrodes. All of the derivatives could be electrocatalytically oxidized at both electrodes. Constant-potential and pulsed-potential amperometry were examined for detection of thiohydantoins following reversed-phase liquid chromatography. Amperometric detection at a fixed potential of 1,2 V vs. SCE resulted in detection limits of less than 10 pmol for lysine and glycine derivatives (50-ul injection). Use of a gradient elution program with a C-18 reversed-phase column allowed separation of nearly all common amino acids as MTH derivatives.

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