Improved Culture Method for the Isolation ofHistoplasma capsulatumandBlastomyces dermatitidisfrom Contaminated Specimens
Open Access
- 2 February 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 63 (2) , 276-280
- https://doi.org/10.1093/ajcp/63.2.276
Abstract
Smith, Coy D., and Goodman, Norman L.: Improved culture method for the isolation of Histoplasma capsulatum and Blastomyces dermatitidis from contaminated specimens. Am. J. Clin. Pathol. 63: 276–280, 1975. Studies were performed to evaluate a method for selective isolation of Histoplasma capsulatum and Blastomyces dermatitidis from contaminated specimens. Ammonium hydroxide placed on an agar medium surface was found to inhibit the growth of many bacteria, yeasts, and saphrophytic fungi normally found in specimens such as animal tissues and sputum. In one study involving the culture of B. dermatitidis from canine tissues, 24% more isolations were obtained on a medium using NH4OH compared with a similar medium. Increases in the isolation of H. capsulatum from sputum specimens were also obtained, ranging from 20 to 32% compared with four other media without NH4OH.Keywords
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