The Use of Benlate for Distinguishing Between Haploid and Diploid Strains of Aspergillus nidulans and Aspergillus terreus

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Abstract
On media containing the fungicide Benlate (benomyl), growth of diploid strains of A. nidulans was interrupted while that of haploid strains remained stable. The difference was shown not to be the result of recessive nonallelic mutations to Benlate tolerance within the haploid strains. The difference provides an easy way of classifying the ploidy of segregants from both sexual and parasexual analyses. The results on A. nidulans were extrapolated to A. terreus.