Seed Storage of Several Horticultural Species in Liquid Nitrogen (-196°C)1

Abstract
Seeds of 14 vegetable and 2 flower species were placed in paper envelopes and submerged in liquid nitrogen (LN2, –196°C) for periods up to 180 days with no adverse effects on germination. Vigor tests with beans (Phaseolus vulgaris L.), lettuce (Lactuca sativa L.), and peas (Pisum sativum L.) also indicated no reduction in germination or vigor after exposure to LN 2. Liquid nitrogen offers promise as a medium for long-term storage of seed germplasm.

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