MEDIATION OF TISSUE EOSINOPHILIA IN HYPERSENSITIVITY REACTIONS .2. SEPARATION OF A DELAYED EOSINOPHIL CHEMOTACTIC FACTOR FROM MACROPHAGE CHEMOTACTIC FACTORS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 31  (2) , 263-271
Abstract
In anaphylactic cutaneous lesions induced by DNP[dinitrophenylated]-Ascaris extract in the guinea-pig, the time-course of delayed tissue eosinophilia paralleled that of the macrophage reaction, reaching its peak in 24 h. Macrophages could be differentiated from lymphocytes by the numerous lysosomal granules which stained for acid phosphatase. Extracts from such skin lesions contained a delayed eosinophil chemotactic factor and 2 different macrophage chemotactic factors. Most of the delayed eosinophil chemotactic factor was separated from the 2 macrophage chemotactic factors by gel filtration on Sephadex G-100 and Sephadex G-200 in that order. The eosinophil chemotactic factor after re-chromatography on Sephadex G-200 showed no or little chemotactic activity for macrophages.