Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.

Publisher Website
Google Scholar
Abstract
A set of 239 heat-sensitive (38.degree.) and 16 cold-sensitive (18.degree.) conditional mutants of T. thermophila was generated by combining techniques to manipulate large numbers of clones with a method for the selection of self-fertilized cells after mutagen treatment. A simple technique is presented for determining the fertility of individual clones; 179 of the clones in this set (71%) are fertile. The fertile conditional mutants had lesions in a number of diverse functions, including nucleic acid metabolism, mobility, cell cycle and cortical pattern.