EOSINOPHIL CHEMOTACTIC FACTOR (ECF) .1. RELEASE FROM POLYMORPHONUCLEAR LEUKOCYTES BY CALCIUM IONOPHORE A23187

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (1) , 229-234
Abstract
Eosinophil chemotactic factor (ECF), previously thought to be primarily associated with human basophils and mast cells, could be released from human neutrophils (PMN [polymorphonuclear leukocytes]) and eosinophils, but not lymphocytes by the Ca ionophore A23187. Release of ECF from PMN was time and dose-dependent. Like antigen-induced, basophil-derived ECF, PMN-derived ECF had a high selectivity for eosinophils as determined by differential counts of migrating cells and by deactivation studies. Chromatographic analysis of PMN-derived ECF on Sephadex G-25 showed an elution pattern very similar to that of basophil-derived ECF. Using rat peritoneal cells, it was shown that mast cells and mast cell-depleted cell preparations could be induced to release an ECF that appears to be similar or identical to human ECF. ECF may play a role in inflammatory processes involving cells other than basophils and mast cells.

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