DEVELOPMENT AND USE OF A QUANTITATIVE IMMUNOASSAY FOR THE CALCIUM-BINDING PROTEIN (MOLECULAR-WEIGHT, 11500) OF MORRIS HEPATOMA-5123

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (3) , 974-979
Abstract
An antiserum was developed against a MW 11,500 Ca-binding protein purified to homogeneity from Morris [rat] hepatoma 5123tc. The antiserum was specific and did not cross-react with an excess of 2 other Ca-binding proteins, calmodulin or parvalbumin. The amount of the tumor protein was quantitated immunologically in several Morris hepatomas, early neoplastic liver nodules and mouse sarcomas. This protein could not be detected in normal rat tissue. The Ca-binding protein in mouse tumors appeared similar to that in rats by crossed polyacrylamide gel electrophoresis-immunoelectrophoresis and crossed isoelectric focusing immunoelectrophoresis.