Simultaneous Determination of Serotonin, 5‐Hydroxyindoleacetic Acid, 3,4‐Dihydroxyphenylacetic Acid and Homovanillic Acid by High Performance Liquid Chromatography with Electrochemical Detection
- 1 March 1982
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (3) , 840-843
- https://doi.org/10.1111/j.1471-4159.1982.tb08708.x
Abstract
A rapid and highly sensitive procedure for simultaneous determination of serotonin, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic acid and homovanillic acid is described. After precipitation of proteins with perchloric acid the samples are applied directly to a high performance liquid chromatograph, with electrochemical detection. As little as 20 pg of serotonin, 5-hydroxyindoleacetic acid, and 3,4-dihydroxyphenylacetic acid and 200 pg of homovanillic acid can be detected. One chromatographic run requires less than 10 min.Keywords
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