Direct Visualization of A-, P-, and E-Site Transfer RNAs in the Escherichia coli Ribosome
- 16 February 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5251) , 1000-1002
- https://doi.org/10.1126/science.271.5251.1000
Abstract
Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction. The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.Keywords
This publication has 25 references indexed in Scilit:
- Recombinant photoreactive tRNA molecules as probes for cross-linking studiesBiochimie, 1994
- The ribosome at improved resolution: New techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particlesUltramicroscopy, 1994
- Functional aspects of ribosomal proteinsBiochimie, 1991
- Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNAJournal of Molecular Biology, 1990
- Direct localization of the tRNA-anticodon interaction site on the Escherichia coli 30 S ribosomal subunit by electron microscopy and computerized image averagingJournal of Molecular Biology, 1988
- Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomesFEBS Letters, 1983
- Distance moved by transfer RNA during translocation from the A site to the P site on the ribosomeJournal of Molecular Biology, 1982
- Crystal structure of yeast phenylalanine transfer RNAJournal of Molecular Biology, 1978
- Idealized atomic coordinates of yeast phenylalanine transfer RNABiochemical and Biophysical Research Communications, 1976
- Three-Dimensional Tertiary Structure of Yeast Phenylalanine Transfer RNAScience, 1974