Specific immunolysis of serotonergic nerve terminals using an antiserum against tryptophan hydroxylase
- 25 March 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 182 (2) , 489-492
- https://doi.org/10.1016/0014-5793(85)80360-4
Abstract
An antiserum to tryptophan hydroxylase purified from whole rat brain when incubated with rat striatal synaptosomes in the presence of complement caused release of 18% of LDH, 20% loss of potassium and 60% loss of tryptophan hydroxylase. Uptake of 5-HT was reduced by 60%. Anti-tryptophan hydroxylase alone, or complement alone were without action. The antiserum plus complement had no effect on DA uptake and did not release TH or GAD. These results suggest selective lysis of serotonergic nerve terminals had occurred. The antiserum plus complement reduced choline uptake by 45%. However, this did not seem due to lysis of cholinergic terminals, as ChAT was not releasedKeywords
This publication has 6 references indexed in Scilit:
- A Sensitive Radiometric Assay for Tryptophan Hydroxylase Applicable to Crude ExtractsJournal of Neurochemistry, 1983
- Specific lysis of GABAergic synaptosomes by an antiserum to glutamate decarboxylaseFEBS Letters, 1983
- Lysis of cholinergic synaptosomes by an antiserum to choline acetyltransferaseFEBS Letters, 1982
- Quantitation of Cholinergic Synaptosomes from Guinea Pig BrainJournal of Neurochemistry, 1981
- A rapid radiochemical method for the determination of choline acetyltransferaseJournal of Neurochemistry, 1975
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951