Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes
- 14 October 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 160 (1) , 37-41
- https://doi.org/10.1042/bj1600037
Abstract
Preparations of yeast cell membranes can catalyze in vitro the N-acetyl-.beta.-D-glucosaminylation of the asparagine sequon at residues 34-36 of bovine pancreatic RNase A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated RNase and analyzed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mg2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.This publication has 16 references indexed in Scilit:
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