Mode of transmission of human T-cell leukemia virus type I (HTLV I) in a human promyelocytic leukemia HL60 cell
- 14 April 1986
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 37 (4) , 601-606
- https://doi.org/10.1002/ijc.2910370420
Abstract
HTLV‐I propagated in IMR90 human diploid fibroblasts was transmitted to human myeloid leukemia HL60 cells at a low efficiency. After co‐cultivation for 3 months, the viral genome was detected in 14/48 HL60 cell clones. Among the 14 HTLV‐I‐infected clones, 8 contained subgenomic fragments alone or in addition to the complete HTLV‐I genome. The frequency of deleted proviruses (9/24 total proviruses) was unexpectedly high. Hirt's supernatant of some of the clones harboring complete HTLV‐I genome(s) in the chromosome contained both linear and circular HTLV‐I proviral DNAs. The circular DNAs were composed of one LTR and 2 LTR closed circular proviruses. These clones produced infectious HTLV‐I constitutively, which was proved by transmission of the viral genome into fresh IMR90 cells by co‐cultivation. However, in these clones, re‐integration of extrachromosomal provirus into their own chromosomes was not observed.This publication has 23 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera.Proceedings of the National Academy of Sciences, 1984
- Productive Infection and Cell-Free Transmission of Human T-Cell Leukemia Virus in a Nonlymphoid Cell LineScience, 1983
- Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay.Proceedings of the National Academy of Sciences, 1983
- Human T‐cell leukemia virus type I: Induction of syncytia and inhibition by patients' seraInternational Journal of Cancer, 1983
- Homology of human T-cell leukaemia virus envelope gene with class I HLA geneNature, 1983
- On the mechanism of retrovirus-induced avian lymphoid leukosis: deletion and integration of the proviruses.Proceedings of the National Academy of Sciences, 1981
- Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds.Proceedings of the National Academy of Sciences, 1978
- Characterization of a New Human Diploid Cell Strain, IMR-90Science, 1977
- Covalently closed circular DNA of avian sarcoma virus: Purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresisJournal of Molecular Biology, 1976