CD1c as a target recognition structure for human T lymphocytes: Analysis with peripheral blood γ/δ cells

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Abstract
It has been shown recently that one γ/δ cell line, termed IDP2, derived from a immunodeficient patient recognizes the CD1c molecule on the surface of target cells. In light of these data, we have tested 43 cloned and 11 polyclonal γ/δ cell lines derived from peripheral blood of 19 donors following nonspecific mitogenic stimulation. In this panel, which included lymphocytes expressing various combinations of γ and δ chains, only one clone, termed J2B7, was found to interact with target cells via a CD1c-dependent recognition pathway. These J2B7 lymphocytes have, like IDP2, a δ chain which results from the frequent V1/J1 rearrangement while they use a distinct Vγ gene segment. The data support the view that the CD1c major histocompatibility complex “class I-like” gene product does not have a pivotal contribution to the repertoire of peripheral blood γ/δ cells in adult individuals.