SYNTHESES OF VIRUS-INDUCED THYMIDINE KINASE AND VIRAL-DNA IN HERPES-SIMPLEX TYPE-1 VIRUS-INFECTED CHICK-EMBRYO FIBROBLASTS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 21  (3) , 177-183
Abstract
Herpes simplex virus type 1, strain Kupka, did not replicate in chick embryo fibroblasts (CEF), but the infection was followed by the development of cytopathic changes. This effect could be abolished by UV irradiation of the virus. Virus-induced thymidine kinase was synthesized in the infected cells reaching a maximum level at 24 h post infection. In the presence of cytosine arabinoside, thymidine kinase synthesis was enhanced. The late (post-replicative) viral function, which turns off thymidine kinase synthesis, was probably expressed in the infected CEF untreated with the drug. HSV type 1 laboratory strains Kupka and KOS induced the synthesis of virus-specific DNA in CEF. But in CEF infected with fresh type 1 virus isolates replication of viral DNA was not observed.

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