Platelet-derived growth factor and transforming growth factor-beta 1 differentially affect the synthesis of biglycan and decorin by monkey arterial smooth muscle cells.
- 1 July 1993
- journal article
- abstracts
- Published by Wolters Kluwer Health in Arteriosclerosis and Thrombosis: A Journal of Vascular Biology
- Vol. 13 (7) , 1026-1036
- https://doi.org/10.1161/01.atv.13.7.1026
Abstract
Platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1), two growth-regulatory peptides with opposite effects on arterial smooth muscle cell (ASMC) proliferation, were examined for their influence on the synthesis of two small chondroitin sulfate/dermatan sulfate proteoglycans (CS/DS PGs) called biglycan and decorin. Quiescent ASMCs treated with either PDGF or TGF-beta 1 for 24 hours increased [35S]sulfate incorporation into biglycan 3.3- and 2.9-fold, respectively, whereas the incorporation of [35S]sulfate into decorin was not significantly affected. Treatment with TGF-beta 1 but not PDGF more than doubled the steady-state level of messenger RNA (mRNA) transcripts hybridizing to a complementary DNA (cDNA) encoding biglycan. Both growth factors had little or no effect on steady-state levels of mRNA transcripts hybridizing to a decorin cDNA. Incorporation of [35S]sulfate into biglycan glycosaminoglycan (GAG) was maximal by 12 to 18 hours after either PDGF or TGF-beta 1 addition. Both PDGF and TGF-beta 1 increased the molecular sizes of biglycan and decorin. This increase was a result of the synthesis of longer GAG chains substituted on the core proteins of both PGs. PDGF but not TGF-beta 1 led to an increase of more than twofold in the ratio of 6'- to 4'-sulfated disaccharides in these newly synthesized GAG chains. These results indicate that PDGF and TGF-beta 1 have specific but different effects on the synthesis of small CS/DS PGs by monkey ASMCs in culture.Keywords
This publication has 48 references indexed in Scilit:
- PDGF ligand and receptor gene expression during repair of arterial injury.The Journal of cell biology, 1990
- TGF-β stimulation and inhibition of cell proliferation: New mechanistic insightsCell, 1990
- Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cellsExperimental Cell Research, 1986
- The biology of platelet-derived growth factorCell, 1986
- The Pathogenesis of Atherosclerosis — An UpdateNew England Journal of Medicine, 1986
- Highly sulfated proteochondroitin sulfates synthesized in vitro by rat glomeruliBiochimica et Biophysica Acta (BBA) - General Subjects, 1985
- Modulation of types I and III procollagen synthesis at various stages of arterial smooth muscle cell growth in vitroExperimental Cell Research, 1984
- Proteoglycans in primate arteries. III. Characterization of the proteoglycans synthesized by arterial smooth muscle cells in culture.The Journal of cell biology, 1983
- Effect of purified growth factors on rabbit articular chondrocytes in monolayer culture. II. sulfated proteoglycan synthesisArthritis & Rheumatism, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970