Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin

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Abstract
Nineteen monoclonal antibodies which bind to native dystrophin in the plasma membrane of frozen muscle sections were obtained using a recombinant fusion protein as immunogen. On Western blots of normal mouse muscle extracts, the antibodies bind specifically to a 400 000 M r protein which is absent from dystrophic mouse (mdx) muscle. At least four distinct epitopes have been identified by cleavage mapping methods. Although the fusion protein contained 25% of the human dystrophin sequence (Cys816‐Asp1747; M r 108 000), most of the monoclonal antibodies (15 out of 19) recognize a single fragment of M r 27 500.