A Non-radioisotopic Anion-exchange Chromatographic Method to Measure the CO2/O2Specificity Factor for Ribulose Bisphosphate Carboxylase/Oxygenase
- 1 January 1996
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 60 (6) , 942-944
- https://doi.org/10.1271/bbb.60.942
Abstract
A non-radioisotopic anion-exchange ion chromatographic method for measuring the carboxylation/ oxygenation specificity (τ) of ribulose 1, 5-bisphosphate carboxylase/oxygenase (RubisCO) is presented. The assay measures the amounts of fixation products at varying [CO2]/[O2] ratios to measure the relative rates of CO2 and O2 fixation reactions. The amount of 3-phosphoglycerate (3-PGA) and phosphoglycolate (PG) in the reaction mixture were measured with a conductivity detector and the specific factor was calculated using the following equations: νc = ([3-PGA] – [PG])/2 and νo = [PG]. By this method, specificity factors for RubisCOs were measured without using radioactive reagents.Keywords
This publication has 1 reference indexed in Scilit:
- d-3-Phosphoglycerate, d-2-Phosphoglycerate, PhosphoenolpyruvatePublished by Elsevier ,1965