Blocking of Acid-fixed Nuclear Binding of Epstein-Barr Virus Nuclear Antigen (EBNA) by Different DNA Species

Publisher Website
Google Scholar
Abstract
Treatment of Epstein-Barr virus-determined nuclear antigen (EBNA) with DNA resulted in blocking of its ability to convert acid-fixed EBNA-negative cell nuclei to an EBNA-positive form. Epstein-Barr virus (EBV) DNA, herpes simplex virus type 2 (HSV-2) DNA and DNA isolated from 3 lymphoblastoid cell lines differed in their potency to block this reaction. EBV DNA was about 3 times more effective than cellular DNA in abolishing the ability of DNA-cellulose-purified EBNA to convert acid-fixed nuclei to the EBNA-positive form; the effect of HSV-2 DNA was of intermediate character. No difference was found between the blocking potency of DNA isolated from EBV-genome-negative Ramos cells and EBV-genome-positive Raji and P3HR-1 cells.