Boron Nutrition of Cultured Tobacco BY-2 Cells. II. Characterization of the Boron-Polysaccharide Complex

Publisher Website
Google Scholar
Abstract
In cultured tobacco BY-2 cells, more than 90% of the cellular boron (B) occurs in the cell wall and a negligible amount of B is detected in the membrane fraction. Nearly 80% of the cell wall B binds to rhamnogalacturonan II (RG-II) to form a borate-dimeric RG-II complex. Mono-meric RG-II is not detected in the cell wall, but it is detected in the extracellular polysaccharides. The complex is reconstituted spontaneously in vitro simply by mixing mon-omeric RG-II and boric acid at pH 4. Germanic acid, which partially substitutes for B in the growth of the B-deprived plants, also induces dimerization of RG-II. These results suggests that B may fulfill its essential function as forming the B-RG-II complex in cell walls.

This publication has 0 references indexed in Scilit: