Intraepidemic variants of influenza virus H3 hemagglutinin differing in the number of carbohydrate side chains
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 120 (3-4) , 289-296
- https://doi.org/10.1007/bf01310484
Abstract
During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrate side chains and by direct sequencing of vRNA. Evidence is presented that variants showing a slower electrophoretic mobility of HA1 had consistently acquired a seventh carbohydrate side chain at Asn 126 in epitope A. All the isolates differed from the reference strain A/Port Chalmers/1/73 by the loss of the oligosaccharide at Asn 81. The field strain A/Dresden/3/71 possessed only 5 oligosaccharides in HA1. These results suggest that changes in glycosylation are an important mechanism in the structural variation underlying antigenic drift of HA.Keywords
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