Important Factors in Islet Cell Transplantation: the Role of Pancreatic Fragments’ Size, pH, Potassium Concentration and Length of Intraportal Infusion

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Abstract
The role of pancreatic fragment size prior to collagenase treatment, pH, K+ concentration and length of intraportal infusion was studied in islet cell transplantation of streptozotocin-induced diabetic adult rats. This study demonstrates that pancreatic fragments of 1 mm prior to collagenase digestion, pH of 7.4, extracellular potassium concentration and 10 min of intraportal infusion are important factors in successful islet cell transplantation of adult rats receiving six donor pancreases for amelioration of chemical diabetes. These factors should be taken into consideration when consistent islet cell transplantation results are to be expected.