Detection of Cysticercus cellulosae Antigens in Cerebrospinal Fluid by Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) and Standard ELISA
- 1 July 1987
- journal article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 37 (1) , 169-173
- https://doi.org/10.4269/ajtmh.1987.37.169
Abstract
The dot enzyme-linked immunoassay and standard enzyme-linked immunoassay were used to detect Cysticercus cellulosae antigens in cerebrospinal fluid of patients suffering with neurocysticercosis. Using the dot enzyme-linked immunoassay, 10 of 17 patients (59%) were positive at a reciprocal titer of 128 (range 128–1,024). In the standard assay, 13 of 17 (77%) were positive (range 128–512). Specificity was 100% in both assays using 48 cerebrospinal fluids from patients with various central nervous system infections. The quantification of antigens in cerebrospinal fluid using standard assay and photometric readings showed a range of 17 to 138 ng/ml. The results indicate that both assays are sensitive, specific, and economical for the diagnosis of neurocysticercosis.Keywords
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