A Micro Method for the Quantitation of Tryptophan in Biological Fluids by HPLC-EC

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Abstract
A micromethod for the quantitation of tryptophan in biological fluids, such as whole blood, RBC, serum, plasma or tissue homogenates using HPLC and electrochemical detection is described. -Methyl tryptophan is used as an internal standard. Ten microliters of the sample are added to a mixture of 50 ul of acetonitrite and 10 ul of internal standard (20 ng/ul). This mixture is then diluted with 180 ul of triethylamine phosphate-buffer (pH 3.0). After centrifugation 25 ul of the sample are injected into HPLC system. Standards containing 100 ng and 200 ng of tryptophan are similarly prepared and 25 ul aliquot injected into HPLC.

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