Determination of local conformational stability in fragment 96-133 of bovine growth hormone by high-resolution proton NMR spectroscopy
- 26 January 1988
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 27 (2) , 802-809
- https://doi.org/10.1021/bi00402a047
Abstract
The specific assignment of resonances in the 400-MHz nuclear magnetic resonance (NMR) spectrum of fragment 96-133 (AII) of bovine growth hormone (bSt) is described. Assignments have been made with homonuclear two-dimensional techniques, in particular that of sequential resonance assignment. Complete assignments were possible for the spin systems of 16 residues out of total of 38 and partial assignments for another 5. Assignment of resonances to either residue type or a class of residue was possible for a number of other spin systems. Analysis of the type of nuclear Overhauser effect (NOE) indicates that segments 96-110 and 130-133 are nonregular stable structures and that the segment 111-127, which putatively spans the .alpha.-helix, is not sufficiently stable to generate NOEs.This publication has 4 references indexed in Scilit:
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