A Cytological and Microchemical Study of Thiobacillus thiooxidans

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Abstract
The cell of T. thiooxidans consists of a gram-negative protoplast (pH of isoelectric point, > 4) containing one or more large vacuoles. When the medium contains elementary sulfur, the vacuolar content gives the reactions of both volutin and sulfur; on Waksman''s thiosulfate medium, only volutin is formed. The protoplasm is surrounded by a cell wall, and the cells are imbedded in slime. The cells are actively motile and provided, each, with a single, thick, terminal flagellum. The bearing of the present study on the mechanism of some of the physiol. processes of the organism and on that of the gram reaction is discussed.