Neuroendocrine gene expression in the hypothalamus:In situ hybridization histochemical studies
- 1 December 1987
- journal article
- review article
- Published by Springer Nature in Cellular and Molecular Neurobiology
- Vol. 7 (4) , 353-366
- https://doi.org/10.1007/bf00733788
Abstract
We have reviewed recent studies in whichin situ hybridization histochemistry (ISHH) was used to investigate the regulation of expression of neurohypophysial peptides and hypothalamic releasing hormones. ISHH is a technique in which the presence and quantity of a specific mRNA can be determined in tissue sections with a high degree of resolution and sensitivity. ISHH has been used to measure changes in cellular levels of mRNAs encoding vasopressin, oxytocin, corticotropin-releasing factor, gonadotropin-releasing hormone, thyrotropin-releasing hormone and somatostatin in response to various physiological challenges. A theme emerging from these studies is that changes in levels of mRNA encoding neuroendocrine peptides reflect changes in biosynthesis and secretion.Keywords
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