Proliferative response of human venous endothelial cells to medium conditioned by human tumour cells
- 1 September 1984
- journal article
- Published by Wiley in Cell Biology International Reports
- Vol. 8 (9) , 731-738
- https://doi.org/10.1016/0309-1651(84)90111-5
Abstract
Serum-free medium conditioned by the prior cultivation of cells of the IPT line (derived from a poorly differentiated carcinoma of the lung) contained material of a high MW which stimulated proliferation in cultures of endothelial cells from human umbilical vein. A response was shown by cells seeded on plastic, gelatin or collagen of type I or type IV, at densities of 25 to 200/mm2, and in media with serum concentrations ranging from 0.5 to 20% v/v. Cells plated on biological substrata showed a greater response than those on plastic.Keywords
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