Posttreatment with Amino Compounds Effective in Prevention of Calcification of Glutaraldehyde Treated Pericardium

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Abstract
An attempt has been made to compare the role of different amino compounds in prevention of calcification of glutaraldehyde (GA)-treated pericardium implanted subcutaneously in the rat for 12 weeks. GA pretreated pericardial samples were postfixed separately with glycine, albumin, gentamicin, glycine + gentamicin, and albumin + gentamicin. Severe calcification was noticed in animals undergoing albumin or gentamicin postfixation. Mild calcification was observed in glycine and albumin + gentamicin postfixed materials whereas calcium was undetectable either chemically or morphologically in GA-pretreated pericardium postfixed with glycine + gentamicin (0.32 +/- 0.13.mg/g calcium, dry wt; von Kossa 0) at the 12th week of implantation in the rat.