Plant growth promoting rhizobacteria: strain identification by restriction fragment length polymorphisms
- 1 April 1990
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 36 (4) , 242-248
- https://doi.org/10.1139/m90-042
Abstract
A genomic library of the Pseudomonas putida strain GR12-2 was screened to identify both genus-universal and strain-specific 8-kilobase inserts. The genus-universal clone (pAM141), in combination with the restriction enzymes EcoRI, PstI, and PvuII, was used to generate unique restriction fragment length polymorphisms for 20 related plant growth promoting rhizobacteria and seven reference strains. Strain restriction fragment length polymorphism profiles based on the genus-universal clone pAM141 allow positive identification of individual pseudomonad strains. The strain-specific clone (pAM227) clearly distinguished the parent strain (GR12-2) from 16 other pseudomonad strains, including 8 P. putida, 7 P. fluorescens, and 1 P. cepacia. pAM227 may be useful for monitoring the fate of the P. putida strain GR12-2 in the environment. Key words: pseudomonads, rhizobacteria, restriction fragment length polymorphism, strain identification.This publication has 11 references indexed in Scilit:
- Cloning, sequencing and expression of a Bacillus bacteriolytic enzyme in Escherichia coliMolecular Genetics and Genomics, 1988
- DNA amplification to enhance detection of genetically engineered bacteria in environmental samplesApplied and Environmental Microbiology, 1988
- DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial CommunityApplied and Environmental Microbiology, 1988
- A Positive Strain Identification Method for Rhizobium melilotiApplied and Environmental Microbiology, 1988
- Differentiation ofPseudomonas syringaepv.tomatofromP. s. syringaewith a DNA Hybridization ProbePhytopathology®, 1988
- Plant Growth-Promoting Rhizobacteria on Canola (Rapeseed)Plant Disease, 1988
- Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putida under gnotobiotic conditionsCanadian Journal of Microbiology, 1987
- Bioassay for studying the role of siderophores in potato growth stimulation by Pseudomonas SPP in short potato rotationsSoil Biology and Biochemistry, 1987
- DNA hybridization probe for the Pseudomonas fluorescens groupApplied and Environmental Microbiology, 1986
- Integration of the plasmid prophages P1 and P7 into the chromosome of Escherichia coliJournal of Molecular Biology, 1979