RAPID ALKALINE TRANSFER OF LOW-MOLECULAR-WEIGHT DNA FROM NUSIEVE GTG AGAROSE GELS

  • 1 September 1990
    • journal article
    • research article
    • Vol. 9  (3) , 260-262
Abstract
The rapid alkaline transfer of high molecular weight DNA from agarose gels to nylon membranes has greatly decreased the time required for setup of Southern transfers (3). This technique has been used to resolve genomic DNA greater than 1000 base pairs by conventional electrophoresis on 1% agrarose gels followed by alkaline transfer to nylon membrane. Now we report that this rapid alkaline method can be used for the transfer of low molecular weight DNA fragments (10 to 1000 base pairs) from NuSieve GTG agarose gels to nylon membrane.