DNA Adenine Methylase Is Essential for Viability and Plays a Role in the Pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae
Open Access
- 1 December 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (12) , 7610-7615
- https://doi.org/10.1128/iai.69.12.7610-7615.2001
Abstract
Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to differentSalmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. Overproduction of Dam significantly attenuated the virulence of these two pathogens, leading to, in Yersinia, the ectopic secretion of virulence proteins (Yersinia outer proteins) and a fully protective immune response in vaccinated hosts. Dysregulation of Dam activity may provide a means for the development of vaccines against varied bacterial pathogens.Keywords
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