Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures

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Abstract
The proteins of tobacco suspension cultures grown in a synthetic medium were separated into protoplasmic, cell-wall-extracted, and cell-wall-residual fractions. Each fraction is described in terms of separation from the cell, hydroxyproline/proline ratio, amino acid composition and susceptibility to enzymic hydrolysis. The hydroxyproline to proline ratios for the 3 fractions were 0.2, 0.7 to 1.2 and 2 to 4, respectively. Proline-C14 was incorporated most rapidly into the protoplasmic, less rapidly into the cell-wall-extracted, and least rapidly into the cell-wall-residual fraction. A portion of the protoplasmic protein turned over rapidly, whereas the cell-wall proteins did not turn over rapidly if at all.