AN IN VITRO STUDY OF CYTOSTATIC DRUG EFFECT ON THE DNA SYNTHESIS IN METHYLCHOLANTHRENE INDUCED MOUSE SARCOMAS. CORRELATION BETWEEN IN VITRO RESULTS AND THE RESPONSE IN VIVO

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Abstract
The present work uses an in vitro test model to study the effect of vinblastine sulphate, melphalan, and cytosine arabinoside on the DNA synthesis of methylcholanthrene induced mouse sarcoma. With this model, we studied the possible correlation between the in vitro results and the response to these cytostatic drugs in vivo. Seven different tumours were tested and at the same time the tumour cell suspension was transplanted to a number of recipients. The in vitro effects of the drugs were measured as differences in incorporation of labelled H3‐thymidine in drug‐containing tubes and in control tubes. The effects of the drugs on the methylcholanthrene‐induced sarcomas varied greatly, from strong sensitivity to resistance. Two days after transplantation, cytostatic drug treatment began and continued for 10 days. Its effect was checked with white blood cell counts. The effect on the in vivo growth of the transplanted tumours was evaluated by registration of the number of palpable tumours on different days in each experimental group. A correlation was found between the in vitro test results and those of in vivo treatment. Its biological significance is discussed.