How Thin Should a Section Be?
Open Access
- 25 September 1957
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 3 (5) , 815-816
- https://doi.org/10.1083/jcb.3.5.815
Abstract
The limitations of Ornstein''s conclusions (Jour. Biophys. and Biochem. Cytol., 2, No. 4, Suppl., 53), regarding the maximum useful thickness of a section for electron microscopy, are discussed in the light of earlier experimental and theoretical'' work by the author, which showed that the attainable resolution is of the order of one-tenth the section thickness. At the usual working voltage of 50 to 60 kv and for a resolution of 20 A the maximum thickness should be of order 200 A. Thicker sections will give such a resolution only if embedding material is removed with the electron beam, or if a much-higher voltage is used.Keywords
This publication has 2 references indexed in Scilit:
- L'utilisation d'un copolymère du groupe des polyesters comme matériel d'inclusion en ultramicrotomieCellular and Molecular Life Sciences, 1956
- A New Embedding Medium for Electron MicroscopyNature, 1956