Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation
- 1 February 1999
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 38 (4) , 720-723
- https://doi.org/10.1364/ao.38.000720
Abstract
We report on three-dimensional autofluorescence spectroscopy obtained from rat skeletal muscle tissue under two-photon excitation by an ultrashort pulsed-laser beam. It is demonstrated that two types of fluorophores within the skeletal muscle tissue can be simultaneously excited with the laser beam at a wavelength of 800 nm. The two fluorophores exhibited unique fluorescence spectral peaks at wavelengths of 450 and 550 nm. These spectroscopic signals can be used to form a three-dimensional image, giving the information about the biochemical makeup of the skeletal muscle tissue.Keywords
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