IMMUNOGLOBULIN EXPRESSION AND SYNTHESIS BY HUMAN HEMIC CELL LINES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (4) , 559-565
Abstract
Human cell lines (26) derived from a variety of lymphoid and non-lymphoid malignancies were investigated for their immunological markers, with special reference to the class of immunoglobulin [Ig] expressed. Of the lines, 25 stained positively for surface Ig; IgD and IgM were the major Ig classes on these cells. Six of the lines were chosen for a study of their Ig synthesis patterns over an 18 h period, and the Ig produced was analyzed on SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis. Patterns obtained from the cell lines were similar to those from normal lymph node lymphocytes and differed markedly from plasma cells. Two of the cell lines had abnormal Ig synthesis patterns, characterized as free L chains in 1 case. The cell lines are evaluated for their usefulness as models of Ig synthesis and analogues of normal and neoplastic states.

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