Relationship between acetylcholine synthesis and its concentration in rat cerebral cortex

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Abstract
. The synthesis of carbon‐14 labelled acetylcholine (14C‐ACh) from carbon‐14 uniformly labelled glucose (U.L. 14C‐glucose) under different conditions has been studied. . Increasing KC1 concentration from 4 mm to 31 mm was associated with a large increase in the amount of 14C‐ACh in the medium and a significant decrease in the amount of 14C‐ACh in the tissue. This effect was observed in slices and minces but not in homogenate. . Increasing KC1 concentration from 4 mm to 31 mm resulted in the release of pre‐formed 14C‐ACh. . Elevated pre‐incubation levels of ACh in slices incubated in 4 mm KC1 medium was associated with a decrease in the formation of 14C‐ACh. . These findings are consistent with the hypothesis that the concentration of ACh in the vicinity of the site of synthesis regulates the rate of ACh formation.