Structure and Mechanism of a Coreceptor for Infection by a Pathogenic Feline Retrovirus
Open Access
- 15 February 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (4) , 2717-2729
- https://doi.org/10.1128/jvi.77.4.2717-2729.2003
Abstract
Infection of T lymphocytes by the cytopathic retrovirus feline leukemia virus subgroup T (FeLV-T) requires FeLIX, a cellular coreceptor that is encoded by an endogenous provirus and closely resembles the receptor-binding domain (RBD) of feline leukemia virus subgroup B (FeLV-B). We determined the structure of FeLV-B RBD, which has FeLIX activity, to a 2.5-Å resolution by X-ray crystallography. The structure of the receptor-specific subdomain of this glycoprotein differs dramatically from that of Friend murine leukemia virus (Fr-MLV), which binds a different cell surface receptor. Remarkably, we find that Fr-MLV RBD also activates FeLV-T infection of cells expressing the Fr-MLV receptor and that FeLV-B RBD is a competitive inhibitor of infection under these conditions. These studies suggest that FeLV-T infection relies on the following property of mammalian leukemia virus RBDs: the ability to couple interaction with one of a variety of receptors to the activation of a conserved membrane fusion mechanism. A comparison of the FeLV-B and Fr-MLV RBD structures illustrates how receptor-specific regions are linked to conserved elements critical for postbinding events in virus entry.Keywords
This publication has 73 references indexed in Scilit:
- Relationship between SU Subdomains That Regulate the Receptor-Mediated Transition from the Native (Fusion-Inhibited) to the Fusion-Active Conformation of the Murine Leukemia Virus GlycoproteinJournal of Virology, 2002
- Classifying reverse transcribing elements: a proposal and a challenge to the ICTVArchiv für die gesamte Virusforschung, 2001
- Specificity in Receptor Usage by T-Cell-Tropic Feline Leukemia Viruses: Implications for the In Vivo Tropism of Immunodeficiency-Inducing VariantsJournal of Virology, 2001
- Receptor Binding Transforms the Surface Subunit of the Mammalian C-Type Retrovirus Envelope Protein from an Inhibitor to an Activator of FusionJournal of Virology, 2001
- Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted ProteinsMicrobiology and Molecular Biology Reviews, 2001
- Identification of Envelope Determinants of Feline Leukemia Virus Subgroup B That Permit Infection and Gene Transfer to Cells Expressing Human Pit1 or Pit2Journal of Virology, 2001
- Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface GlycoproteinJournal of Virology, 2001
- [20] Processing of X-ray diffraction data collected in oscillation modePublished by Elsevier ,1997
- Dissection of a retrovirus envelope protein reveals structural similarity to influenza hemagglutininCurrent Biology, 1995
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994