Target-Assisted Iterative Screening Reveals Novel Interactors for PSD95, Nedd4, Src, Abl and Crk proteins
- 1 June 2002
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 19 (6) , 1015-1029
- https://doi.org/10.1080/07391102.2002.10506805
Abstract
A new in vitro screening method has been developed and applied to commercial phage-dis- played cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as targets. 12 novel putative and 2 previously reported interactions were identified in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), is discussed as an alternative platform to existing technologies for pair-wise characterization of protein-protein interactions.Keywords
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