Pitx1 and Pitx2c are required for ectopic cement gland formation in xenopus laevis
Open Access
- 23 July 2001
- Vol. 30 (3) , 144-148
- https://doi.org/10.1002/gene.1051
Abstract
Summary: The mucus secreting cement gland is the anterior‐most ectodermal organ of the Xenopus embryo. The homeobox genes Pltx1 and Pitx2c are expressed in the cement gland primordium. Misexpression of both genes induced ectopic cement gland tissue in whole embryos and transcription of the marker genes Xag1 and Xag2 in animal cap explant cultures. Antisense morpholino oligonucleotides against Pitx1 and Pitx2c inhibited ectopic cement gland formation induced by otx2. Gene knock downs generated by morpholino oligonucleotides were specific and could be rescued by coinjection of Pitx mRNAs. These data demonstrate for the first time the requirement of specific genes for cement gland formation by loss‐of‐function experiments. genesis 30:144–148, 2001.Keywords
Funding Information
- DFG (BL 285/4-1)
- Volkswagenstiftung
This publication has 18 references indexed in Scilit:
- The Pitx2 Homeobox Protein Is Required Early for Endoderm Formation and Nodal SignalingDevelopmental Biology, 2001
- Xotx5b, a new member of the Otx gene family, may be involved in anterior and eye development in Xenopus laevisMechanisms of Development, 2000
- Coincidence of otx2 and BMP4 signaling correlates with Xenopus cement gland formationMechanisms of Development, 2000
- Pitx2 isoforms: involvement of Pitx2c but not Pitx2a or Pitx2b in vertebrate left–right asymmetryMechanisms of Development, 2000
- Xpitx-1 : a homeobox gene expressed during pituitary and cement gland formation of Xenopus embryosMechanisms of Development, 1999
- Anterior specification of embryonic ectoderm: the role of the Xenopus cement gland-specific gene XAG-2Mechanisms of Development, 1998
- Pre-MBT patterning of early gene regulation in Xenopus: the role of the cortical rotation and mesoderm inductionMechanisms of Development, 1998
- The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4Cell, 1996
- The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryosDevelopmental Biology, 1988
- Determination of the dorsal-ventral axis in eggs of Xenopus laevis: Complete rescue of uv-impaired eggs by oblique orientation before first cleavageDevelopmental Biology, 1980