Estimation of cell damage in monolayer culture using 14C-leucine incorporation.

Abstract
A comparison was made of 14C-leucine incorporation and cell viability of T24 human bladder carcinoma cells after treatment with Thio-Tepa, a potent alkylating agent. It was found that Thio-Tepa produced concentration-dependent damage in these cells and also that 14C-leucine incorporation cor-related well with cell viability. 114C-leucine incorporation can be used as a reliable and rapid screening test for the assessment of the relative potencies of anticancer drugs.

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