Isolation of neutral heteropolysaccharide containing mucoprotein from bovine Achilles tendon with the aid of collagenmucoproteinase

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Abstract
With the aid of collagenmucoproteinase, a polysaccharide-containing fraction mucoid2, can be isolated from Achilles tendon. The data of purification and fractionation are reported. The mucoid2 contains protein-bound polysaccharides and hexosamine. Besides mucoid2 the enzyme digests show the presence of hyaluronidase-resistant acid mucopolysaccharides which can be separated by paper electrophoresis and show a very strong meta-chromatic reaction with toluidine blue. It is suggested that mucoid2 may be one of the stabilizing factors of collagen fiber and that it is responsible for the periodic acid-Schiff reaction.