Fluorescent Indicators for Imaging Nitric Oxide Production
- 26 October 1999
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 38 (21) , 3209-3212
- https://doi.org/10.1002/(sici)1521-3773(19991102)38:21<3209::aid-anie3209>3.0.co;2-6
Abstract
The membrane‐permeating indicator DAF‐FM DA is transformed by intracellular esterases into the highly water‐soluble dye DAF‐FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location of NO.This publication has 0 references indexed in Scilit: