INDUCTION OF SUPPRESSOR MACROPHAGES IN MICE BY FUSARENON-X

  • 1 January 1982
    • journal article
    • research article
    • Vol. 47  (4) , 701-708
Abstract
Fusarenon-X, a mycotoxin produced by Fusarium nivale Fn 2B, injected i.p. into BALB/c mice, depressed polyclonal antibodiy formation of mouse splenic lymphocytes in response to pokeweed mitogen (PWM). This inhibitory activity was found in the surface Ig-negative spleen cell fraction of Fusarenon-X-treated mice, sIg-(FX), which comprised mainly T lymphocytes and smaller number of non-lymphocytic cellular elements. Reconstitution experiments for in vitro antibody formation provided evidence that T lymphocytes from Fusarenon-X-treated mice, T(FX), which were separated from non-lymphocytic cells by use of carbonyl iron/magnet, were as effective as T lymphocytes from normal mice, T(N), in supporting antibody formation. Addition of non-lymphocytic cells, or adherent cells prepared from spleen cells of Fusarenon-X-treated mice to normal spleen cells strongly inhibited in vitro antibody formation against PWM [pokeweed mitogen] or a bacterial lipopolysaccharide. Fusarenon-X apparently induced non-lymphocytic suppressor cells in the spleen of the treated mice which had features in common with activated macrophages.

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