ESTABLISHMENT OF A NEW EPSTEIN-BARR VIRUS IMMORTALIZED CELL-LINE FROM CHRONIC LYMPHOCYTIC-LEUKEMIA WITH TRISOMY OF CHROMOSOME-12 THAT PRODUCES MONOCLONAL IGM AGAINST A SHEEP RBC ANTIGEN

  • 1 January 1988
    • journal article
    • research article
    • Vol. 71  (1) , 9-12
Abstract
Leukemia cells from a patient with chronic lymphocytic leukemia (CLL) were found to bind sheep RBC (SRBC) through their monoclonal surface IgM. A lymphoblastoid cell line was obtained by immortalization of leukemic cells with Epstein-Barr virus (EBV). Cultured leukemic cells were found to have a supernumerary chromosome 12, an abnormality typical of CLL of the B cell type. To our knowledge, this is the first EBV-immortalized cell line from B-CLL cells of known SRBC specificity and the third reported CLL cell line carrying trisomy of chromosome 12.

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