Development of an Ultrasensitive Immunoassay for Rapid Measurement of Okadaic Acid and Its Isomers
- 20 August 1999
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (19) , 4198-4202
- https://doi.org/10.1021/ac9901642
Abstract
This report highlights the characteristics of an okadaic acid immunoassay with limits of detection in the subfemtomole range. Two different immunoassay formats were investigated and their characteristics compared in relation to linear ranges, limits of detection, and cross-reactivity with other seafood toxins present in water and/or mussel samples. The developed ELISA system can be manipulated to quantitatively measure total diarrhetic shellfish poisoning (DSP) content or for okadaic acid and dinophysistoxin-1 individual concentrations by variation of the format of the immunoassay. Real mussel samples were validated in percentage recovery test. Calibration curves were established, and aliquots of real samples were tested. Very good recoveries were attained, highlighting the validity of the ELISA system to accurately determine the DSP concentration in mussel samples.Keywords
This publication has 5 references indexed in Scilit:
- A Fluorescent Microplate Assay for Diarrheic Shellfish ToxinsAnalytical Biochemistry, 1997
- Optimization of sample cleanup procedure for determination of diarrhetic shellfish poisoning toxins by use of experimental designJournal of Chromatography A, 1997
- Isolation of dinophysistoxin-2 and the high-performance liquid chromatographic analysis of diarrhetic shellfish toxins using derivatisation with 1-bromoacetylpyreneJournal of Chromatography A, 1996
- Ion‐spray mass spectrometry of marine toxins II. Analysis of diarrhetic shellfish toxins in plankton by liquid chromatography/mass spectrometryRapid Communications in Mass Spectrometry, 1990
- Comparison of three different assays for the assessment of ciguatoxin in fish tissues: Radioimmunoassay, mouse bioassay and In vitro guinea pig atrium assayToxicon, 1982