Studies on the endo-enzymes, particularly the peptidases, of Clostridium histolyticum

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Abstract
Solns. obtained by disrupting C. histolyticum suspensions with a supersonic oscillator contained a dipeptidase, with a pH optimum of 7.6, and a polypeptidase acting optimally at pH 8.7. The polypeptidase was activated by Mg++, while the dipeptidase was unaffected. The prepns. contained also enzymes which attacked various amino acids with liberation of NH3. All of the above enzymes were endo-enzymes, and were not secreted by the intact bacteria.