Abstract
The in vitro synthesis of 11-ketotestosterone and 11β-hydroxytestosterone, from adrenosterone and testosterone, by gonadal and interrenal slices and by sperm was studied. The results establish that adrenosterone is the major precursor of 11-ketotestosterone in both interrenal and testicular tissue. 11β-Hydroxytestosterone was synthesized mainly from testosterone by gonadal and interrenal tissue. The production of 11β-hydroxytestosterone by normal gonadal tissue is reported for the first time.

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